Frequency study of JC virus in patients with Hodgkin and Non- Hodgkin lymphoma

BACKGROUND AND ABJECTIVEThe John Cunningham virus (JCV) is a small, non-enveloped virus with a circular double-stranded genome. It is classified as a pandemic infection and belongs to the Polyomaviridae family. JCV can cause a subclinical infection that results in lifelong latency. However, the latent form of the virus can become active in individuals with immune system deficiencies. JCV is known to induce cellular transformation in human B lymphocytes by interacting with various proteins, such as p۵۳, retinoblastoma family proteins, and cell cycle regulators. Recently, researchers have linked JCV to several types of cancer, including Hodgkin’s and non-Hodgkin’s lymphoma, as well as reactive lymphoma.The primary aim of this study was to analyze the frequency of the JC virus in paraffin samples got from patients diagnosed with Hodgkin’s and non-Hodgkin’s lymphomas, as well as reactive lymphoma.MATERIALS AND METHODSFor this study, ۹۰ paraffin samples were collected from patients diagnosed with Hodgkin’s, non-Hodgkin’s, and reactive lymphoma from the pathology department and each group contained ۳۰ samples. the tissue was deparaffinized, and its DNA was separated. The real-time PCR method was employed to amplify the JC virus-specific primer in order to detect the virus DNA. To achieve a higher level of accuracy, a Nested PCR technique was implemented.RESULTS AND DISCUSSIONAfter analyzing the patient characteristics, we observed that the mean age was ۳۳, ۵۰, and ۴۶ in the Hodgkin group, non-Hodgkin group, and reactive group, respectively. Nevertheless, the results of the study indicated that the real-time PCR and nested PCR did not detect any signals in the sample, which demonstrated that there was no JC virus present.CONCLUSIONThe absence of the JC virus in the sample suggests that the infection caused by the virus alone may not be responsible for the development of lymphomas. It is suggested that JCV can cause cancer through the hit-and-run mechanism, in which the presence of the virus is not necessary.Other molecular factors and mechanisms may also contribute to the occurrence of lymphomas in humans.

کلیدواژه ها:

Hodgkin ، Non- Hodgkin lymphoma ، Reactive lymphoma ، JC Virus