Identification of probiotic bacteria isolated human colostrum milk and invitro investigation their antimicrobial effect on the infectious agents of children diarrhea

BACKGROUND AND OBJECTIVESBreast milk is an important factor in the initiation and composition of the neonatal flora gut microbiota which contains potentially probiotic lactic acid bacteria. Lactic acid bacteria are very significant to human health due to the production of some antimicrobial substances and ability to inhibit pathogenic bacteria. There is an increasing trend in the field of research into the characterization of new acid lactic isolates with potential application to the health and disease prevention. Accordingly, the present study isolated probiotic bacteria from human colostrum milk to analyze their antimicrobial potential on agents of children diarrhea.MATERIALS AND METHODSIn this study, isolates were assayed to phenotypic characteristics, in vitro viability different temperatures and high salt concentration, resistance to gastrointestinal simulated conditions, the antimicrobial activity of antibiotics and as well, their antimicrobial activity were surveyed on ۴ of diarrheagenic bacteria, Escherichia coli ۰۱۵۷:H۷ (ATCC ۴۳۸۹۰), Staphylococcus aureus (ATCC ۲۵۹۲۳), Salmonella typhi (PTCC۱۶۰۹) and Shigella dysentery (ATCC ۱۳۳۱۳).RESULTS AND DISCUSSIONBased on the results, ۴ of isolates from ۶ lactobacilli isolates and ۷ isolates from ۳۴ Enterococci isolates had capacities survive under the mentioned conditions. Through molecular identification, ۲ Lactobacillus isolates belonged to L. fermentum species and the other ۲ isolates belonged to L. plantarum and L. salivarius species. The most inhibitory effect was observed in C۱ isolate from enterococci and Q۲b (L. fermentum) isolate from lactobacilli on enterohemorrhagic E. coli and S. aureus, respectively.CONCLUSIONThe identified lactic acid bacteria as probiotics could be used in formula infant to protect children health versus infectious diseases such as diarrhea after the legal procedures and to get necessary safety.

کلیدواژه ها:

Antimicrobial effect ، Colostrum ، Diarrhea ، Lactic acid bacteria ، Probiotic