Fabrication and evaluation of the antibacterial effects of nano-graphene films containing vancomycin compared to vancomycin

BACKGROUND AND OBJECTIVESBacterial resistance is one of the significant medical problems over the past decades. A large number of diseases caused by bacterial agents and the development of multidrug resistance in genes have raised the need to produce new drug molecules or novel drug delivery systems for better treat diseases. Novel drug delivery systems emerged in which inorganic compounds of metal oxide nanoparticles such as silver, gold, copper, titanium, and zinc have shown remarkable antibacterial activity. Adding nanoparticles to a polymeric material increases the likelihood that they will affect bacterial activity. As mentioned above, this study aimed to fabricate nanographene films containing vancomycin and investigate their antibacterial effects.MATERIALS AND METHODSGram-positive Staphylococcus aureus bacteria were identified by mannitol salt agar culture medium and coagulase test. FT-IR, SEM, thermal gravimetric analysis (TGA), and differential thermal analysis (DTA) were used to detect the morphology of the film and to identify the constituent elements. Also, to investigate the antibacterial effects of the films, the disk diffusion method was used. Based on the inhibition zone, the growth inhibition was calculated and compared to the control.RESULTS AND DISCUSSIONExamining the results of the inhibition zone, it was observed that the graphene oxide disk alone does not have significant antibiotic properties. Strong antibacterial effects were observed in vancomycin discs. Graphene oxide-vancomycin complex disks also showed significant antibiotic effects on various strains of S. aureus.CONCLUSIONIt was observed that the formation of graphene oxide-vancomycin complex in some strains of S. aureus has even more controlled antibiotic effects compared to vancomycin alone.

کلیدواژه ها:

Vancomycin ، Nanoparticles ، Graphene Oxide ، Disc Diffusion