Plasmid-mediated ciprofloxacin-resistance genes in Pseudomonas aeruginosa isolated from hospitalized patients in Zabol, Iran

BACKGROUND AND OBJECTIVESPlasmid-dependent resistance to ciprofloxacin is increasingly spreading among Pseudomonas aeruginosa isolates worldwide. In this study, the evaluation of antibiotic resistance to ciprofloxacin and the presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA and aac (۶′)-Ib-cr genes in Pseudomonas aeruginosa isolated from hospitalized patients in Zabol was investigated.MATERIALS AND METHODA total of ۱۰۳ clinical isolates of Pseudomonas aeruginosa were collected from hospitalized patients. The bacterial isolates were identified through standard laboratory protocols. Antimicrobial susceptibility was determined by the standard disk diffusion method. The presence of qnrA, qnrB, qnrC, qnrD, qnrS, qepA, and aac (۶′)-Ib-cr genes was investigated by the polymerase chain reaction (PCR) method and sequencing.RESULTS AND DISCUSSIONOverall, ۳۷ (۳۵.۹۲%) isolates were non-susceptible to ciprofloxacin. The prevalence rates of qnr genes were as follows: qnrA (۱۳.۵۱%), qnrB (۲۷.۰۲%), qnrS (۵۴.۰۵%) and aac (۶′)-Ib-cr (۸۳.۷۸%). The qnrC, qnrD, and qepA, genes were not recognized in any isolates.CONCLUSION:The expansion of ciprofloxacin-resistant plasmid genes plays an important role in the prevalence of Pseudomonas aeruginosa ciprofloxacin-resistant strains.

کلیدواژه ها:

Antimicrobial resistance ، qnr genes ، Pseudomonas aeruginosa