Investigating antibiotic resistance pattern and the presence of sea and seb genes in Staphylococcus aureus isolated from creamy pastry in Ilam

The sweeter than a favorite human food, which has increased the consumption in many countries in recent decades. On the other hand, enterotoxins of Staphylococcus aureus are important factors in bacterial pathogenesis. Among them, enterotoxin A and B are known as the main cause of staphylococcal food poisoning in the world. Although the disease is mild and self-limiting and the mortality rate in this type of poisoning is low, but it is considered as one of the most important poisonings, in terms of health- economy, around the world. Recently, antibiotic resistance in bacteria has been considered a major public health problem. Food is an important factor in transmitting resistance to antibiotic agents. Residues of veterinary drugs in food can be carcinogenic, mutagenic, odd, allergenic, or toxic in humans. The presence of this bacterium or its toxins in food can be a potential risk to human health.One hundred samples (cream bread and roulette) were taken from confectioneries in Ilam. Of the total ۱۰۰ samples, ۳۲ isolates (۳۲%) were detected by biochemical tests as Staphylococcus aureus (۲۳ isolates from roulette and ۹ isolates from cream bread).In this study resistance to tetracycline, erythromycin and oxacillin antibiotics in Staphylococcus aureus isolates from creamy pastry was checked by disk diffusion method. Also the presence of sea and seb genes by molecular method using PCR was screened.In total of the isolates, ۵ isolates (۱۷.۸۵%) was positive for sea gene, three isolates (۱۰.۷۱%) for seb gene and two isolates (۷.۱۴%) was positive for both genes. The study of antibiotic resistance of isolates also showed that on average ۸۸% were resistant to tetracycline, ۸۷% to erythromycin and ۴۳% to oxacillin. The two antibiotics tetracycline and erythromycin had the highest resistance among isolates. The lowest resistance was related to gentamicin and imipenem. The results of the present study show a close relationship between erythromycin and tetracycline antibiotic resistance genes and sea gene.

کلیدواژه ها:

Antibiotic resistance ، Staphylococcus aureus ، Enterotoxin A and B ، femA ، sea ، mec A and seb genes