Cellulose production from bacteria isolated from rotten fruit jam

BACKGROUND AND OBJECTIVESThe purpose of this study was to isolate and identify bacteria capable of producing bacterial cellulose and to optimize the conditions for the production of bacterial cellulose that can be useful for commercialization and industrial applications.MATERIALS AND METHODSIn this study, the bacterial isolates obtained from rotten blackberry jam. A sample of rotten blackberry jam was prepared and cultured on Hestrin-Schramm's agar medium. In this study, a strain with maximum ability to produce cellulose was isolated from rotten jam samples and identified as Rhodococcus sp. After the purification of cellulose, enzymatic digestion by cellulase enzyme was used to confirm the presence of cellulose. Optimization studies were carried out for process parameters such as inoculum density, temperature, pH, stirring and carbon and nitrogen sources.RESULTS AND DISCUSSIONThe strain produced ۲.۴۳ g/L cellulose in ۶ days under optimal growth conditions of temperature (۳۰ ∘C), pH (۶.۰), glucose (۲%), yeast extract (۰.۵%) and peptone (۰.۵%). These findings are for continuous improvement of cellulose synthesis by Rhodococcus sp. for the production of cellulose on an industrial scale. Scanning electron microscopy also showed that the cellulose produced by this bacterium had a fibrous structure and micron-sized pores.CONCLUSIONThe results of the present study showed that Rhodococcus strains have the ability to produce cellulose. Cellulose produced by bacteria is preferred for use in various industries due to its higher purity than plant cellulose. The product obtained from this bacterium can be used as a cosmetic and medical material.

کلیدواژه ها:

Bacterial cellulose ، Optimization strategies ، Applications