Adherence inhibition by anti-Oma۸۷ antibodies of Acinetobacter baumannii to A۵۴۹ cell line

BACKGROUND AND OBJECTIVESAcinetobacter baumannii is a nosocomial pathogen that has stunned the world and poses a significant threat to the global healthcare system, most notably in ventilator-associated pneumonia, bloodstream infections, urinary tract infections, and meningit. Adhesion to target cells is essential for a pathogen. This is established with the expression of virulence factors. A. baumannii stems from its ability to adhere and colonize biotic and abiotic surfaces, employing a change of strategy currently dubbed ‘persist and resist’ rather than the traditional toxin expression of other pathogens. This, coupled with an equally confounding capacity to survive in normally unfavorable conditions makes A. baumannii a formidable pathogen.MATERIALS AND METHODSrOma۸۷ was expressed, purified, and injected into groups of BALB/c mice. The antibody titer was measured by the indirect ELISA. Adhesion and internalization of A. baumannii strains were studied in A۵۴۹ cell line.RESULTS AND DISCUSSIONOma۸۷, a conserved and potent immunogen of A. baumannii, significantly inhibited adhesion to and internalization in A۵۴۹ cells of A. baumannii strains.CONCLUSIONMonovalent vaccines containing conserved antigens offer a satisfactory level of protection against A. baumannii infections.

کلیدواژه ها:

Acinetobacter baumannii ، Adherence ، Cytotoxicity ، Oma۸۷ ، Internalization