Protective effects of probiotic mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on carbon tetrachloride-induced damage in kidney tissue of male Wistar rats

BACKGROUND AND OBJECTIVESCarbon tetrachloride (CCl۴) by producing free radicals causes damage in various tissues, including the kidney. In this study, the protective effect of probiotic mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on tetrachloride-induced damage on kidney tissue of male rats was investigated..MATERIALS AND METHODSIn this experimental study, ۲۱ male rats (weight range ۲۰۰-۲۲۰ g) were randomly divided into ۳ groups of ۷ including: control group, carbon tetrachloride group (۲ mg/kg )and receiving carbon tetrachloride + probiotic mixture (Lactobacillus casei, Lactobacillus Rhamnosus and Lactobacillus heloticus( CFU/Ml۱۰۹(. CCl۴ was by intraperitoneal injection and receiving probiotics for ۳۵ days by gavage method.After the end of the treatment period; to measure serum levels of creatinine, total protein and albumin, blood was taken from the heart area. Then, the kidneys were removed from the animals for histological examination. Data analysis in different groups was done with SPSS software and one-way variance statistical test, and P<۰.۰۵ was considered significant.RESULTS AND DISCUSSIONCCl۴ injection to rats significantly increased the serum creatinine level compared to the control group(P<۰.۰۰۱), while it decreased the serum albumin and total protein levels. Also, treatment with probiotic mixture significantly caused significant changes in the receiving group compared to the carbon tetrachloride receiving group(P<۰.۰۱). Treatment with probiotic mixture caused improvement in the histopathological changes of kidney tissue sections.CONCLUSIONThe results of this study showed that the probiotic mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) can moderate the toxic effects caused by carbon tetrachloride in the kidney, which is probably due to the antioxidant function of lactic probiotics.

کلیدواژه ها:

Probiotic ، Carbon tetrachloride ، Serum urinary factors ، Kidney ، Rat