Investigating the effect of Bifidobacterium bifidum supernatant on mrkD gene expression of Klebsiella pneumoniae resistant to carbapenem and producing biofilm

BACKGROUND AND OBJECTIVESBacterial resistance to antibiotics is one of the most important threats to human health. Klebsiella pneumoniae is one of these highly resistant bacteria that increase pathogenicity and higher resistance to antibiotics and host defense by forming a biofilm, and on this basis, the use of probiotics has been considered. We aim to determine the anti-biofilm and antibacterial effects of the supernatant isolated from Bifidobacterium bifidum on carbapenem-resistant Klebsiella pneumoniae and to determine the effects of the supernatant isolated from Bifidobacterium bifidum in reducing the expression of genes involved in the colonization and biofilm formation of Klebsiella pneumoniae resistant to carbapenems.MATERIALS AND METHODS۳۰ isolates of Klebsiella pneumoniae available in the archive of the laboratory were studied. A ۲۴h culture was prepared from the stocks, and Klebsiella pneumoniae strains were confirmed with IMViC tests, and carbapenemase-producing strains were determined with mCIM, eCIM, and MHT tests. PCR was used to check the presence of the mrkD gene; Supernatant was prepared from Bifidobacterium bifidum and biofilm, antibiofilm, and MIC tests were performed, and finally Real-time PCRRESULTS AND DISCUSSIONWe found that the effect of the supernatant of a probiotic (Bifidobacterium bifidum) on about ۷۰% of Klebsiella pneumoniae strains with the mrkD gene and resistance to carbapenem decreases the expression of the mrkD gene.CONCLUSIONIn this study, we found that the use of probiotics can partially help to combat antibiotic resistance in carbapenem-resistant Klebsiella pneumoniae bacteria.

کلیدواژه ها:

Klebsiella Pneumoniae ، Bifidobacterium Bifidum ، Mrkd ، Carbapenemase ، Probiotics