Synthesis and characterization of magnesium nanoparticles loaded with lactoferrin and evaluation of its antibacterial properties

BACKGROUND AND OBJECTIVESNanoparticles enhance infectious illness diagnosis and therapy. Antibacterial nanoparticles are intriguing because of their tiny sizes, high surface-to-mass ratios, and other peculiar physical and chemical properties. Metal oxide-based nanoparticles may swiftly infiltrate bacterial cell walls, increasing ROS generation and blocking cell wall enzymes. Lactoferrin (LF) has antibacterial properties as it is preventing bacteria from obtaining a critical food supply. Therefore, LF has ability to kill germs. In this study, we aimed to synthesis and characterize magnesium nanoparticles (MgNPs) loaded with LF and evaluate their antibacterial properties.MATERIALS AND METHODSThe sol-gel technique was used to manufacture the MgNPs. Surface modification was then used to load LF. Physical characteristic of Mg-LF such as particle size, zeta potential and homogeneity were analyzed by zatasizer. Standard agar diffusion and minimum inhibitory concentration (MIC) tests were used to find out how well LF-loaded Mg NPs worked against Gram-negative bacteria like Pseudomonas aeruginosa.RESULTS AND DISCUSSIONOur results demonstrated the successful synthesis of LF-loaded MgNPs with an average size of ۱۸-۲۰nm. Mg nanoparticles have a negative charge and LF is trapped by Mg nanoparticles because it has a positive charge. The LF-loaded MgNPs exhibited no significant antibacterial activity against Pseudomonas aeruginosa, as evidenced observed in the agar diffusion assay. MIC values revealed no effective bactericidal activity in Pseudomonas aeruginosa strains.CONCLUSIONIn conclusion, LF-loaded magnesium nanoparticles were created. The Bradford test and Zetasizer results were showed that and their properties were analyzed Mg NPs were efficiently entrapped LF by electrostatic interactions and inhibited that from protease degradation. But against other researches LF-loaded MgNPs was not had any antibacterial activity, because MgNPs were changed LF structure that was confirmed by UV-Visible and Fluorescence spectroscopy.