Bordetella pertussis CyaA toxin through regulating RHO GTPases pathway in human can cause whooping cough: In silico study

BACKGROUND AND OBJECTIVESBordetella pertussis is an aerobic gram-negative coccobacillus and the reason of whooping cough. This bacterium causes disease exclusively in humans. It has shown bacterial proteins by interacting to human proteins cause many diseases. This interaction can change the signaling and metabolic pathways and cellular processes of infected host. Therefore, to identify molecular mechanism of Bordetella pertussis pathogenesis we decided to use bioinformatics software to find bacterial proteins that bind human proteins and then evaluated effected pathways.MATERIALS AND METHODSThe analysis was done with bioinformatics software. To identify bacterial motifs that interact with human proteins, ImitateDB database was used. In this database, a strain of Bordetella pertussis was selected and motifs interacting with human cells were identified. Host interactor protein IDs were extracted from this database, and these codes were converted into gene symbols through BioDBnet database, and the function of these proteins was determined using string and EnrichR database.RESULTS AND DISCUSSIONImitateDB database showed Bifunctional adenylate cyclase toxin-hemolysin (CyaA) that plays a crucial role in host colonization can interact by CALM۱ which is members of the EF-hand calcium-binding protein family. CALM۱ also via different motif interact by ۹ human proteins as figure ۱ shown. As you can see from figure ۲ Functional analysis by EnrichR indicated these genes significantly regulated RHO GTPases pathway. RHO GTPases pathway is key regulators involved in cell morphology, cell-matrix adhesion and cytoskeletal reorganization.CONCLUSIONIt is recommended to use more bacteria and bioinformatics software to identify different bacterial proteins that interact with human proteins and by examining the function of each protein, the way to predict and know more about the methods of causing disease by bacteria and prevention and let’s treat diseases more smoothly.

کلیدواژه ها:

Bordetella pertussis ، Bioinformatics analysis ، ImitateDB database ، BioDBnet database ، string database ، EnrichR database