Evaluating the bactericidal Activity of a disinfectant agent on surfaces

BACKGROUND AND OBJECTIVESHospital-acquired infections (HAIs) are a widespread issue, particularly in developing countries, meanwhile Surfaces play an important role in transmission of diseases. The main purpose of infection control in hospitals is to prevent the spread of infections. Disinfection and sterilization are essential for preventing the transmission of Pathogenic microorganisms to patients. This article has emphasized the necessity of surface sterilization procedure. The chemical sterilizing agent used in study was Steril-C, which produces Peracetic acid (PAA). PAA is based on low-temperature sterilization technology. The aim of this study is assessment of disinfection effect of PAA on surface.MATERIALS AND METHODSThis study was accomplished in order to evaluating disinfection activity of PAA for surfaces. E.coli is a major cause of nosocomial infections so in this study E.coli ATCC ۲۵۹۲۲ was used and prepared a working culture. Then the test suspension containing ۱.۵×۱۰۸ to ۵×۱۰۸ cfu/ml of E.coli was made. The test suspension and interference substance and PAA was mixed to evaluate disinfection activity of PAA. To negative control of test suspension, distilled water was used instead of PAA. For preparing validation suspension, test suspension and diluent was mixed and then cultured.RESULTS AND DISCUSSIONPAA disinfectant solution was able to reduce more than ۶ log number of microorganisms and there were no visible colonies on the blood agar culture medium. PAA is a powerful disinfectant with a wide spectrum of antimicrobial activity. Previous studies demonstrated that the residual bacterial number of the PAA-disinfected endoscope was significantly lower than Glutaraldehyde and Ortho-Phthalaldehyde disinfected endoscopes.CONCLUSIONPeracetic Acid is able to destroy all the microorganisms in this suspension, thus this agent can be used as a high-level disinfectant with powerful effect. Therefore Peracetic Acid can be appropriate to disinfection of medical surfaces.

کلیدواژه ها:

PAA ، disinfectant ، surface ، E.coli ، infection control