Interaction of Gut Microbiota and Their Metabolites in patients with sepsis

BACKGROUND AND OBJECTIVESSepsis is a complex clinical disorder with heterogeneous etiological factors. Given its high mortality rate, it is considered a global health issue. Recently, the link between gut microbiota and their metabolites, especially short-chain fatty acids, in the pathophysiology of sepsis has been reported. However, there are few findings to confirm this relationship. This study aimed to evaluate some key gut microbiota members, pathogenic bacteria, and short-chain fatty acids in non-ICU patients with sepsis caused by bacteremia compared to a control group.MATERIALS AND METHODSIn this case-control study, ۴۵ stool samples from patients with sepsis and ۱۵ healthy persons were collected from October ۲۰۲۱ to August ۲۰۲۲ in Tabriz, Iran. The position of some gut microbiota members and the main short-chain fatty acids concentration were assessed in the two groups by the Q-PCR and the high-performance liquid chromatography system.RESULTS AND DISCUSSIONFaecalibacterium prausnitzii and Bifidobacterium spp. As bacterial with protective features in non-ICU patients with sepsis decreased significantly. Moreover, the concentrations of acetic acid and propionic acid significantly decreased in this group compared to the healthy volunteers. In contrast, the pathogenic bacteria members such as Enterobacteriaceae and Bacteroides spp. Increased significantly in the patients compared to the healthy individuals. The concentration of butyric acid decreased in the patients, but this change was not significant in the two groups.CONCLUSIONProtective and immune functions of F. prausnitzii and Bifidobacterium spp., as well as acetate and propionate, are evident. In this investigation, this profile was significantly reduced in non-ICU patients with sepsis compared to the control group.

کلیدواژه ها:

Gut microbiota ، Sepsis Short-chain fatty acids ، Faecalibacterium prausnitzii ، Bifidobacterium spp.