Isolation and introducing Candida SP. PZ۹ is a resistant strain to high concentrations of zinc and its potential for practical use in food supplements

Trace elements cannot be obtained in sufficient quantities from food alone to meet the human body's nutritional demands, resulting in a constant state of inadequacy of these elements in the body. Because inorganic zinc is challenging for mammals to absorb and utilize in the gastrointestinal tract, it is preferable to use the organic form. This study aims to enrich zinc in Candida sp. PZ۹ produces yeast rich in zinc and uses it in the food industry and animal and human feed.A total of ۵۰ samples of yeast were examined to determine their capacity for growth in varying concentrations of zinc. One strain that displayed a high level of resistance to high zinc concentrations was chosen after conducting screening tests. This particular strain was sent for molecular identification, which revealed it to be Candida SP. PZ۹ is a type of yeast that can withstand zinc concentrations of up to ۴۰۰۰ ppm. following the identification, the yeast was cultured and its dry weight and atomic absorption were measured. The measurements of dry weight at a concentration of ۲۰۰۰ ppm, ۳۰۰۰ ppm, and ۴۰۰۰ ppm of zinc, were ۱۴.۵ g L-۱, ۸ g L-۱, and ۴.۵ g L-۱. In addition, according to the AAS results, the sample contained ۱۹۰۰ ppm of zinc, indicating that the yeast cells had absorbed this amount of the zinc metal, which shows the high ability of metal absorption by this yeast. Moreover Candida SP. PZ۹ grows well in medium containing concentrations of ۱۰۰۰ ppm and ۲۰۰۰ ppm of other metals such as copper, selenium, and chromium.Due to the mentioned properties of this yeast in absorbing zinc, high biocompatibility, and easy absorption, upon obtaining the necessary approvals, it has the potential to be used as a nutritional supplement for animal and human feed.

کلیدواژه ها:

Biosorption ، Bioaccumulation ، Enrichment yeast ، Food supplements