Lipid production from a newly isolated yeast Candida parapsilosis EBL۲۹ for biodiesel production

Fossil fuels are the main source of energy demand; however, climate changes have made a great concern to look for new and efficient renewable energy sources. Oleaginous yeasts can produce and store lipids at least ۲۰% of their cell dry weight in the form of extracellular or intracellular single-cell oil (SCO). In the recent research, fifty-three yeast strains were screened and isolated from different environmental samples. Then, samples were cultured on Rose Bengal Chloramphenicol agar for microscopic observation of colonies. Thirty-four strains were able to lipogenesis on Oleaginous Yeast Enrichment Medium containing ۵% glycerol and subsequently incubated on saponification wastewater to investigate dry biomass and lipid production. Among these strains, the lowest cell dry weight (CDW) was ۴.۵ gr/L and ۱.۲۷ gr/L lipid production. The most promising results with ۱۳.۵ gr/L cell dry weight and ۶.۷۵ gr/L lipid production, was related to Candida parapsilosis EBL۲۹ that was identified by ITS sequencing with ۹۹.۴۷% homology to Candida parapsilosis ATCC ۲۲۰۱۹. With its ۵۰% lipid yield (lipid production to CDW ratio), Candida parapsilosis EBL۲۹ can be a potential candidate to produce the third generation of biofuels using inexpensive industrial wastewater as a growth medium.

کلیدواژه ها:

Biodiesel ، Biofuel ، Lipid extraction ، Lipogenesis ، Oleaginous yeasts ، Renewable energy