Effect of endophytic fungi isolated from Rhabdociadium aucheri on Aspergillus Flavus

Endophytes are organisms that live in living plant cells. The relationship they establish with the plant varies from symbiosis to the border of pathogenicity. Endophyte means "inside the plant". The using of this word includes a wide range of organisms such as bacteria, fungi, and insects inside plants. They can colonize in any organ of the host. Endophytic fungi are often referred to as asymptomatic fungi that can be in all plants. The genus Rhabdosciadium is grouped in the family Apiaceae (Umbelliferae), order Apiales, order Magnoliopsida, suborder Rosidae and branch Magnoliophyta. This genus has ۵ species in the world. The species of this genus are known as Shaleel in Iran. Aspergillus flavus is a pathogenic fungus that has a wide distribution in most of the world. This species is known for its symbiosis on cereals, legumes and nuts. This fungus is also an opportunistic pathogen of human and animals and causes aspergillosis in people with immune system deficiency. Therefore, the aim of this study is to investigate the effect of endophytic fungi isolated from R. aucheri on A. flavus. In order to investigate the effect of endophytic fungi isolated from R. aucheri on A. flavus, in this observational study, after isolating the endophytic fungi on the pathogenic fungus A. flavus, the effect and then the percentage of inhibition was measured. The experiment was conducted as a randomized complete block design with ۳ replications. Data analysis was carried out with SPSS ۱۸ software and the comparison of means was done with Duncan's multi-range test method at the probability level of ۵%. Finally, it was found that cross-cultivation of Alternaria rosae K۱۱ against A. flavus with The inhibition rate of ۸۷.۵% indicates the highest inhibitory power and also the cross-culture of Alternaria radicina ۲K۲ against A. flavus with inhibition rate of ۱۲.۱% indicates the lowest inhibitory power of this endophyte against A. flavus.

کلیدواژه ها:

Endophyte ، Secondary metabolite ، Fungi ، Rhabdosciadium aucheri ، Microorganism