Inhibitory effect of Thyme and Citrus honeys on the biofilm formation of amulti drug-resistant Pseudomonas aeruginosa isolate

BACKGROUND AND OBJECTIVESPseudomonas aeruginosa is an opportunistic human pathogen with high ability to formantibiotic-resistant biofilms. Honey has been used since ancient times for its nutritional andmedicinal properties, especially for its antimicrobial activity. In this study the effect ofThyme and Citrus honeys on the biofilm formation of a resistant strain of P. aeruginosa wasinvestigated.MATERIALS AND METHODSA multi drug-resistant strain of P. aeruginosa which was isolated from the urinary tract of apatient referred to Imam Reza Hospital was used in this study. The primary assay by discdiffusion method showed resistance of this bacterium against cefazolin, ceftizoxime,ampicillin, ciprofloxacin ,nitrofurantoin, cefepime, and clavulanic acid. The effect ofdifferent concentrations (۱۰۰-۷۰% v/w) of Thyme honey as well as Citrus honey on bacterialgrowth and its biofilm formation capacity was assessed by the well-diffusion and microtiterplatemethods, respectively.RESULTS AND DISCUSSIONThe result obtained from the well-diffusion method showed that ۱۰۰% concentration ofThyme and Citrus honeys inhibited the growth of multi drug-resistant P. aeroginosa withinhibition zone diameters of ۱۵ and ۲۰ mm, respectively. Statistical analysis results obtainedfrom the microplate reader showed that all the concentrations of both honeys show theinhibition effect on biofilm formation of recruited P. aeroginosa isolate.CONCLUSIONThe study revealed antibacterial and antibiofilm activities of Thyme and Citrus honeysagainst multidrug-resistant P. aeruginosa which can causes devastating acute and chronicinfections in individuals with compromised immune systems. This result suggests that thesehoneys might find a place in clinical practice, alone or as part of combination antimicrobialtherapies, to treat multidrug-resistant bacteria.

کلیدواژه ها:

Honey ، Pseudomonas aeruginosa ، Biofilm