The antibacterial effects of Lactobacillus reuteri isolated from yogurts against pathogenic Helicobacter pylori

BACKGROUND AND OBJECTIVES Lactobacillus reuteri is a well- known probiotic with beneficial effects to human health. This species can be found in different organs of the body such as skin, breast, gastrointestinal tract, and urinary tract. Due to its antimicrobial effects, the beneficial effect against Helicobacter pylori which is a pathogen in the human gastrointestinal tract can be tested. H. pylori causes infection in the stomach by attaching to gastric epithelial cells. If H. pylori receptors get blocked by Lactobacillus reuteri receptors, H. pylori bacteria cannot be able to attach to gastric epithelial cells and this will prevent gastric diseases. Therefore, the main aim of this study was to determine the antimicrobial properties of L. reuteri against H. pylori.MATERIALS AND METHODS To doing so, different samples of yogurts around Iran were collected, then ۱gr of each samples weighted and solved in ۱۰ mL Saline, then ۱۰ mL of this was added to ۹۰ mL of MRS broth, following that were cultured on MRS agar and incubated to reach the colonies, each of colonies were identified and characterized by staining, biochemical and sequencing analysis to determine the species. After culturing the H. pylori, the co-aggregation and antibacterial effects were studied.RESULTS AND DISCUSSION The sequencing results revealed that the isolated species was L. reuteri and coaggregated strongly with H. pylori with large numbers of co-aggregation clumps formed at the bottom of the tube and a clear upper suspension. The results of co-aggregation showed that there is ۹۱.۸% co-aggregation between L. reuteri and H . Pylori. Moreover, the disc diffusion results showed significant inhibitory effects of L. reuteri against H. Pylori.CONCLUSION In summary, based on these results it can be found that the L. reuteri which is a probiotic can be applied in therapeutic application for treatment of disease related to H. pylori in gastrointestinal tract.

کلیدواژه ها:

Probiotic ، Co-Aggregation ، L.Reuteri ، H.Pylori