Histopathological investigation in the tissues of mice following the injection of alpha Clostridium perfringens type A toxin

BACKGROUND AND OBJECTIVESClostridium perfringens (C. perfringens) is one of the most pathogenic species in the genus Clostridium that produces many toxins. This bacterium is divided into different types (A to G) based on the main toxins secreted. C. perfringens type A causes the destruction and damage of the cell membrane due to its ability to secrete alpha toxin, followed by vascular, muscular, and hemolytic damage as intestinal bleeding syndrome, acute intestinal inflammation, enterotoxemia, and sudden death in ruminants.MATERIALS AND METHODSAt first, different strains of C. perfringens type A isolates were cultured in nutrient media. In order to reconfirm using molecular methods and performing Multiplex PCR using specific primers, the presence of the alpha gene in the samples was confirmed. Genotypic examination and minimal lethal dose (MLD) test with different dilutions of bacterial suspension supernatant were performed in mice. After necropsy and sampling, sampling was done for histopathological studies.RESULTS AND DISCUSSIONThe results showed that there was an infiltration of lymphocytes with degeneration in the liver tissue. Also, epithelial lesions observed in the brain and kidney tissues were observed. A significant difference was observed in the examination of histopathological results.CONCLUSIONIt concluded that alpha toxin can cause lesions in the tissues, which causes the subsequent pathogenesis of C. perfringens type A poisoning.

کلیدواژه ها:

Hemorrhagic syndrome ، Clostridium perfringens ، Toxin