Prevalence of Human Papilomavirus by molecular method about clients of Arad Medical diagnostic Laboratory in Kerman city, Iran

INTRODUCTIONHuman Papillomavirus (HPV) is an oncogenic DNA virus with more than ۲۰۰ types. It is estimated to be the most common sexually transmitted infection and its prevalence is the highest among young persons within the first few years after sexual debut. The important Low-risk (LR) HPV types are (۶, ۱۱, ۴۰, ۴۲, ۴۳, ۴۴, and ۵۴), but the most important High-risk (HR) HPV types are (۱۶, ۱۸, ۳۱, ۳۳, ۳۵, ۳۹, ۴۵, ۵۱, ۵۲, ۵۶, ۵۸, and ۵۹). More than ۵۰% of cervical cancers are related to HR-HPV۱۶. It is also effective in enhancing the incidence of cervical intraepithelial neoplasia (CIN) grades I, II, III. Accordingly, the aim of this study was to evaluate the prevalence of HPV by molecular method in Kerman.MATERIALS AND METHODSThis descriptive epidemiological research was conducted during spring ۲۰۲۳ about clients of Arad Medical Diagnostic Laboratory, Kerman, Iran. Pap smears were taken from women with ۲۰ to ۶۰ years old. DNA extraction was performed related to ۲۰۰ samples by Semi-automatic extraction machine using the relevant kit instructions. Extracted DNA of the HPVs were monitored (Genotyping) by real-time PCR method to detect ۳۴ different types of HPVs by using ۴ types of probes.RESULTS AND DISCUSSIONThe proportion of high-risk (HR)-HPV was ۸۵.۷۱%. The most common HR-HPV types were HR-HPV۵۱ (۳۷%), HR-HPV۵۶ (۲۴%), HR-HPV۵۹ (۱۳%), HR-HPV۱۶ (۷.۸%), HR-HPV۵۲ (۵.۲%), and HR-HPV۱۸ (۵.۲%). The only low-risk (LR)-HPV type was observed as LR-HPV۶ (۷.۸%). Three patients had also colposcopy test which one of them showed dysplasia of CIN۱.CONCLUSIONA large-scale screening of HR populations is very important and critical. We believe that HPV vaccines, regular education and physical examinations are significant impacts on the prevention of HPV-related diseases. This research includes an updates and highlight on the prevalence of HPV types related to the women of Kerman (South of Iran) during spring of ۲۰۲۳.

کلیدواژه ها:

Human papilloma virus (HPV) ، Real time PCR ، Genotype ، Cervix cancer