Investigating the effect of Lactobacillus brevis probiotic bacteria lysate on the growth rate of the AGS cell line

BACKGROUND AND OBJECTIVESProbiotics demonstrate functional effects as anti-inflammatory, antioxidants, immune system boosters, antibacterial, anti-diabetic, and anti-cancer. One of these probiotics is lactobacillus brevis, which is able to cause the induction of apoptosis through the effect on the mitochondrial signaling pathway. The purpose of this research is to evaluate the effects of lactobacillus brevis lysate on the growth rate of the AGS cell line after twenty-four hours of cell implantation.MATERIALS AND METHODSLactobacillus brevis was obtained from the Center for genetic resources and approached CFU/mL ۳/۵-۴/۹ ×۱۰۹ after implantation. Then, the bacteria were separated using a centrifuge to extract their lysate using a new Sonication method. The obtained lysate was lyophilized to determine the density and ۲, ۱, ۰/۵, ۰/۲۵, and ۰/۱۲۵ mg/mL were the results. The AGS cell line was cultured and transferred to plate ۹۶ which contained the bacteria’s lysate to check cell viability with the MTT technique after exposure to lysate for twenty-four hours.RESULTS AND DISCUSSION.The results illustrate that the viability of the cell was in the lowest density (۰.۱۲۵ mg/mL) for ۷۵% and in the highest density (۲mg/mL) for ۴۴%. It can be concluded that increasing the density causes a lack of growth in cells.CONCLUSIONThe lysate of the probiotic Lactobacillus brevis reduces the survival of cancer cells of the AGS cell line. Therefore, it may be used to control and reduce the growth of cancer cells.

کلیدواژه ها:

Lactobacillus brevis ، AGS cell line ، lysate ، MTT assay