Inhibitory effect of jujube and barberry honeys on the biofilm formation ofCandida albicans

BACKGROUND AND OBJECTIVESJujube honey originated from jujube tree shows anti-inflammatory and antibacterial effects.Barberry honey obtained from Berberis spp. (Berberidaceae) is commonly used to treatdiarrhea and heal wounds.The aim of this study was to investigate the effect of pure jujubeand barberry honey on the inhibition of biofilm formation by the resistant strain of Candidaalbicans which over time, scientists implemented diverse strategies to overcome.MATERIALS AND METHODSTwo strains of Candida albicans, O۳۱ and G۱۰۹ that were isolated from the patients underendoscopy from Imam Reza Hospital were obtained to be used in this study. The effect ofdifferent concentrations (۱۰۰-۷۰% v/w) of jujube and barberry honey on the yeasts growthand their biofilm formation ability was assessed by the well-diffusion and microtiter-platemethods, respectively.RESULTS AND DISCUSSIONIn the well-diffusion assay although no clear inhibition zones were observed, at ۱۰۰%concentration of both honeys, the growth of recruited yeasts were significantly decreasedcompare to control. After execution of microplate-titer method and consequent statisticalanalyses, the results revealed that no biofilm was formed in the presence of all examinedconcentrations of both honeys.CONCLUSIONThe study exposed antifungal and anti-biofilm activities of jujube and barberry honeysagainst both strains of Candida albicans which is a common cause of infection of the skin,oral cavity and esophagus, gastrointestinal tract, vagina and vascular system of humans. Thisresult suggests that these honeys might find a place in clinical practice, alone or as part ofcombination antimicrobial therapies, to treat emerging resistant Candida albicans species.

کلیدواژه ها:

Honey ، Candida albicans ، Biofilm