Optimization of Spirulina algae culture medium by response surface method (RSM)

BACKGROUND AND OBJECTIVESSpirulina algae (Arthrospira platensis) has gained considerable attention for its nutritional benefits and diverse industrial applications. To harness its full potential, optimizing the culture medium is essential for promoting robust growth and maximizing biomass productivity. This study focuses on culture medium optimization using RSM and the exploration of mixotrophic culture to further enhance Spirulina’s productivity.MATERIALS AND METHODSThe study employed a systematic approach to optimize the culture medium for Spirulina algae using RSM. A Box Behnken Design (BBD) was implemented to investigate the interactive effects of critical factors on Spirulina growth and biomass accumulation. Key parameters including NaNO۳, NaCl and K۲HPO۴ were investigated in this experiment and the experiments were designed with the help of Minitab Statistical software (V۲۱). Next, biochemical parameters including biomass, protein, etc. were measured.RESULTS AND DISCUSSIONThe RSM-optimized culture medium enhanced Spirulina's growth and biomass productivity. The interactive effects of the key factors were analyzed, leading to the identification of the optimal nutrient composition for sustained cultivation. The optimized culture medium resulted in higher biomass yield and enriched biochemical content, including proteins and pigments contributing to its value as a nutritious food source. Mixotrophic conditions stimulated enhanced growth rates and metabolic activities; It can also increase the risk of contamination of the cultivation environment.CONCLUSIONThe comprehensive study demonstrated the significance of employing RSM in optimizing the Spirulina culture medium to maximize growth and biomass productivity. The integration of sustainable nutrient sources, such as organic carbon in mixotrophic culture, further enhanced Spirulina's potential as a valuable microorganism. These findings contribute to the advancement of Spirulina cultivation techniques, fostering its integration into diverse sectors and promoting a more sustainable future.

کلیدواژه ها:

Optimizing culture media ، Spirulina algae ، Random surface methodology (RSM) ، Mixotrophic culture