BACKGROUND AND OBJECTIVESTularemia is an important zoonosis caused by Francisella tularensis. The serological tests such as enzyme-linked immunosorbent assay (ELISA), are widely used for tularemia diagnosis. The classical ELISA tests rely on lipopolysaccharides (LPS) of the bacterium as antigens, which requires culturing live strains of F. tularensis in the laboratory and can lead to false-positive results due to cross-reactions with other gram-negative bacteria. To address these limitations, the researchers propose using recombinant proteins as antigens for tularemia serodiagnosis. This study aimed to identify immunodominant antigens of F. tularensis that posed strong immunological properties and diagnostic value based on a literature review and conducting a bioinformatics analysis on them.MATERIALS AND METHODSWe conducted a literature review among the articles in PubMed and Scholar. In this research, immunodominant antigens that posed strong immunological properties and diagnostic value were selected among the studies. Finally, six proteins including FTT۰۰۷۷ (۲-oxoglutarate dehydrogenase component E۲, succinyltransferase dihydrolipoamide), FTT۰۹۷۵ (conserved hypothetical protein), FTT۰۹۷۵ (conserved hypnotical protein), FTT۱۶۹۶ (chaperone ۶۰ (GroEL; ۵۷ kDa), FTT۱۲۶۹c (DnaK chapronin), FTT۰۵۸۳ (FopA (outer membrane associated protein) and FTT۰۴۷۲ (Acetyl-CoA carboxylase, biotin carboxyl carrier protein subunit) that were more immunodominant selected for this purpose. Then, bioinformatics studies were conducted to assess their heterogeneity, indicating that these genes are conserved.RESULTS AND DISCUSSIONThe present study identified six immunodominant F. tularensis antigens through proteomics, microarray, and immunoblotting techniques including FTT۰۰۷۷, FTT۰۹۷۵, FTT۱۶۹۶, FTT۱۲۶۹c, FTT۰۵۸۳, and FTT۰۴۷۲ based on literature review. The selected antigens, including FTT۰۰۷۷, FTT۰۹۷۵, FTT۱۶۹۶, FTT۱۲۶۹c, FTT۰۵۸۳, and FTT۰۴۷۲ showed a heterogeneity rate ranging from <۰.۶۳ to <۰.۳۱, indicating that they are conserved genes and have the potential as diagnostic targets. The use of these recombinant proteins in the diagnosis of tularemia is suggested as they are more immunodominant and elicit an immune response.CONCLUSIONThis study suggests that using these six recombinant proteins in the diagnosis of tularemia can overcome the limitations of current methods and provide a new strategy for accurate and early detection of the disease. Further research and validation studies are needed to establish the effectiveness and reliability of these proteins in the diagnosis of tularemia.