The Study of the Genes Causing Resistance to Aminoglycosides in Escherichia coli Strains Isolated from Clinical Specimens

Escherichia coli is the most common cause of urinary tract infection (UTI), with a prevalence of ۸۰%. Enzymatic inactivation of antibiotics of the aminoglycoside family by aminoglycoside-modifying enzymes is the primary mechanism of E. coli resistance to these pharmaceutical drugs, and the recent expression of the Metylase ۱۶SrRNA gene in Gram-negative bacteria that cause high levels of resistance to these antibiotic medications are considered to be a significant concern. This study aimed to determine the antibiotic resistance pattern and the prevalence of antibiotic resistance genes against aminoglycoside antibiotics among clinical isolates of E. coli by PCR.About ۵۰۰ clinical E. coli isolates were recovered in ۲۰۱۷ from the hospitals of Ilam. Antibiotic susceptibility testing was determined against selected antibiotics using the disk diffusion method and E-test according to CLSI standards. The prevalence of antibiotic-resistance genes among clinical E. coli isolates was determined using PCR. Data were analyzed using SPSS software and the Chi-square test. A p-value less than ۰.۰۵ is considered statistically significant.E-Test results showed that ۷۲.۴% and ۷۱.۳% of isolates were resistant to Tobramycin and Gentamicin, respectively. Also, ۷۵(۷۹.۸%) and ۹۱(۹۶.۸%) of E. coli isolates contained aac (۳)-IIa, and aac.(۶)-Ib, but other used genes were not detected in any isolate. Due to Gentamicin's increased use and availability, its resistance was higher than other antibiotics in many areas, such as Ilam. However, the frequency of resistance to these antibiotics generally varies from region to region, implying the need for antimicrobial susceptibility testing before treatment.

کلیدواژه ها:

Escherichia coli ، Aminoglycoside-modifying enzymes ، Gene ، PCR