The antibacterial effect of an environmentally-isolated specific bacteriophage belonging to Myoviridae family in combination to polymyxin B against multidrug-resistant Acinetobacter baumannii clinical isolates

BACKGROUND AND OBJECTIVESThe multidrug-resistant Acinetobacter baumannii (A. baumannii) has re-emerged as one of the predominant causes of hospital infections causing failure in infection eradication. Phage therapy has recently received attention, particularly against multidrug-resistant (MDR) infections. The aim of this study was assessment of the effect of specific bacteriophages against MDR-A. baumannii clinical isolates.MATERIALS AND METHODSIn this study, a specific bacteriophage was isolated from environment and identified morphologically using transmission electron microscopy (TEM). Additionally, MDR-A. baumannii were isolated and their susceptibility to single bacteriophage and in combination with polymyxin B was investigated.RESULTS AND DISCUSSIONIsolated bacteriophage (Ap۳۸) had a polyhedral head (۱۰ ± ۸۵ nm) and short tail (۵۰ ± ۵ nm), belonging to Myoviridae family. The bacteriophage contained a collar at the end of the head. The simultaneous use of phage and polymyxin B had significantly higher antibacterial activity than single phage against A. baumannii.CONCLUSIONTherefore, the use of phage therapy in combination to antibiotics can be a promising approach for the elimination of drug-resistant infections.

کلیدواژه ها:

Bacteriophage ، Phage therapy ، Multidrug resistance ، Acinetobacter baumannii