Investigating the level of aflatoxin M۱ in Sarshir by ELISA in Karaj city

BACKGROUND AND OBJECTIVESDairy products are mainly susceptible to contamination with aflatoxin M۱ (AFM۱), which can cause harmful effects such as hepatotoxicity, mutagenicity, toxigenicity, teratogenicity, carcinogenicity, neurotoxicity and cell toxicity, as well as suppressing the immune system and neoplasticity. Considering the people's acceptance of the consumption of dairy products such as Sarshir, as well as the significant risks of AFM۱ in consumers, the investigation of contamination with this substance in traditional Sarshir dairy products was carried out in different areas of Karaj city.MATERIALS AND METHODS۴۰ samples of Sarshir products from traditional yogurt shops from different parts of Karaj city (northwest, northeast, southeast and southwest) from each region, ۱۰ samples were purchased in sterile containers, ۱۰۰ grams each. The samples were transferred to the laboratory at a temperature of ۴ degrees Celsius and up to ۲۴ hours, and then they were evaluated for aflatoxin M۱ contamination using the ELISA methodRESULTS AND DISCUSSIONThe obtained results indicate that aflatoxin M۱ in Sarshir samples in different areas of Karaj city is significantly lower than the permissible limit (۱۰۰ppt). The lowest value was ۱۲ ppt and the highest value was ۳۲ ppt. (Mean = ۱۴.۷۰ ± ۳.۴۹ ppt). The no significant difference is observed between amount of aflatoxin in Sarshir samples from the defferent places of Karaj (p>۰.۰۵).CONCLUSIONAccording to the results obtained from this study, the amount of aflatoxin in Sarshir samples collected from Karaj city was lower than the permissible limit. Monitoring the way this product is prepared and distributed, as well as checking the amount of aflatoxin in the final product, can be effective in preventing possible contamination. The way to prepare Sarshir and the steps used in it (including the selection of raw milk, the containers used, the amount of boiling, its chemical and microbial characteristics) can be a reason for reducing the amount of aflatoxin in this product.

کلیدواژه ها:

Aflatoxin ، Sarshir ، Karaj City ، Traditional Dairy Product