Vitamin K۲ screening in Bacillus spp. isolated from soil samples of Tehran, Golestan, Markazi and Mazandaran provinces

Vitamin K۲ (menaquinone, MK-n) is one of the three types of vitamin K, which produced by bacteria (۱, ۲). It has an important role to prevents osteoporosis and cardiovascular disease (۲). Insufficient intake in food sources has made researches valuable to achieve high vitamin K۲ producer strains (۳, ۴, ۵). In present study, soil Bacillus populations screened for menaquinone production potency. Ten soil sample collected from different part of central and northern regions of Iran. In the investigation of ۹۷ isolated strains for vitamin K۲ production through liquid state fermentation in specific media, the vitamin concentration measured in ۲۴۸nm UV-spectroscopy following extraction by n-hexane: ۲-propanol (۶). Analysis approved through potent menaquinone producer isolates by thin layer chromatography (TLC) and menaquinone structure determined by liquid chromatography- mass spectrometry (LC-MS) in Superior isolates. Their chromatogram showed Menaquinone-۷ and menaquinone-۱۲ contents in JM۹ and MG۲ extracts and menaquinone-۷ by PK۱۳. They characterized by gram straining and ۱۶SrRNA sequencing (۷); respectively Bacillus cereus JM۹, Bacillus cereus MG۲ and Bacillus albus PK۱۳ It was the first report of isolation Bacillus cereus and Bacillus albus producing vitamin K۲ from soil origin. Due to the key role of bacteria in the biological production of vitamin K۲, superior isolates with natural capability are valuable. They can be optimized for higher quantities and applied as candidate for future biotechnological application.

کلیدواژه ها:

Menaquinone ، Bacteria ، Optical Density ، Chromatography