Investigating the effects of postbiotic mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on the antioxidant factors of testicular tissue infected with Escherichia coli bacteria in rat

BACKGROUND AND OBJECTIVESProbiotics have several properties depending on the strain. Some probiotics play an important role in preventing infection and balancing the immune system due to the interaction between the intestinal mucosa and cells in the immune system. This study aims to investigate the properties of probiotic mixture (Lactobacillus casei, Lactobacillus rhamnosus and Lactobacillus heloticus) on the antioxidant factors of testis tissue infected with Escherichia coli bacteria in rats.MATERIALS AND METHODSIn this experimental study, ۲۱animals were divided into three groups, including the control group, infected with Escherichia coli bacteria (۱۰۸ CFU/ml) and the infected model + receiving probiotics (۱۰۹ CFU/ml). Induction of infection was done by intraperitoneal injection and receiving probiotics for ۳۵ days by gavage method.After the treatment and dissection of the animals, the testis tissue was extracted to evaluate the antioxidant factors total antioxidant capacity( TAC),total antioxidant status(TOS) ,Malondialdehyde(MDA), Catalase(Cat)in different groups. Data analysis in different groups was done with SPSS software and one-way variance statistical test and P<۰.۰۵ was considered significant.RESULTS AND DISCUSSIONThe results showed a decrease in TAC, Catalase and an increase in TOS, MDA in the group infected with Escherichia coli bacteria compared to the control group (P<۰.۰۰۱). Also, the change in the level of antioxidants in the group treated with probiotic mixture was significantly compared to the infected group.CONCLUSIONThe present study showed that the protective effect of probiotic mixture on Escherichia coli infection of testicular tissue antioxidant factors may be related to the antioxidant function and free radical scavenging of proboscis.

کلیدواژه ها:

Probiotic ، Antioxidant ، Escherichia coli ، testis ، rat