Phenotypic detection of efflux pump in Pseudomonas aeruginosa and Staphylococcus aureus isolates from burn wound infection

BACKGROUND AND OBJECTIVESSome bacteria showed resistance to beta-lactamase after some time. Efflux pumps play a very important role in creating antibiotic resistance of Staphylococcus aureus and Pseudomonas aeruginosa strains. This study aims to investigate microbial resistance and phenotypic investigation of efflux pump in Pseudomonas aeruginosa and Staphylococcus aureus strains isolated from burn patients.Recent studies suggest that efflux pumps may be used by the cell as a first-line defense mechanism to avoid lethal drug concentrations until .MATERIALS AND METHODSPhenotypic investigation of efflux pump by Cardwell method: In order to investigate the activity of efflux pump phenotypically, all isolates were investigated using agar technique containing ethidium bromide by Cardwell method.RESULTS AND DISCUSSIONIn the present study, ۲۰ wound samples of burn patients were infected with Pseudomonas aeruginosa and ۱۰ samples were infected with Staphylococcus aureus. Staphylococcus aureus was resistant to ampicillin, penicillin and cefazolin ۱۰۰% and cefotaxime, levofloxacin, ciprofloxacin, ۷۰%, ۸۰%, ۹۰%, respectively, and Pseudomonas aeruginosa was resistant to imipenem, ciprofloxacin, levofloxacin, ceftazidime, ۸۵%, respectively. ۶۵%, ۵۵%, ۸۵% were resistant. ۶۰% Pseudomonas aeruginosa produced ESBL, ۷۰% Staphylococcus aureus produced beta-lactamase. ۷۵% and ۷۰% of MDR isolates of Pseudomonas aeruginosa and Staphylococcus aureus had efflux pumps, respectively.CONCLUSIONThe findings indicate that most of the isolates collected from patients with severe burns include strains of Staphylococcus aureus and Pseudomonas aeruginosa, and most isolates of Pseudomonas aeruginosa and Staphylococcus aureus showed multidrug resistance and produced beta-lactamase enzymes. It was also shown that most of the isolated bacterial population with multi-drug resistance and beta-lactamase enzyme production have an efflux pump, so it is important to investigate the presence of this pump in proposing a suitable treatment model for patients infected with these bacteria. Considering the presence of the efflux pump in Pseudomonas aeruginosa and Staphylococcus aureus strains, it is important to investigate the presence of these genes in proposing a suitable treatment model for patients infected with these bacteria in medical centers.

کلیدواژه ها:

Antibiotic Resistance ، Pseudomonas Aeruginosa ، Staphylococcus Aureus ، Efflux Pump