Molecular investigation of the contamination of some recreational waters in Mazandaran province with BK and JC viruses

BACKGROUND AND OBJECTIVESWater is considered as the most vital component in the origin of life. Water can be contaminated by various industrial toxic and inorganic pollutants as well as pathogens. Contaminated water is responsible for the transmission of various diseases and is considered the main cause of death of approximately ۵۰۲,۰۰۰ people worldwide each year. Viruses cause ۱۰ to ۱۰,۰۰۰ times more disease risk than bacteria. Human polyomaviruses JCV and BKV are good candidates because they are commonly found in environmental water samples from different geographical regions with relatively high abundance. Polyomaviruses are excreted in urine and can spread through water.MATERIALS AND METHODSThis research was conducted on ۶۰ samples of recreational water in Mazandaran province (pool, pond, river, sea). Human polyomaviruses JCV and BKV were isolated by filtration method using filters (۰.۲۲ and ۰.۴۵). Specific primers were designed for BKV and JCV, and the molecular method of polymerase chain reaction (PCR) were detected.RESULTS AND DISCUSSIONHuman polyoma virus JCV and BKV in recreational water samples based on ۶۰ analyzed samples, JCV was detected in ۲۳ cases (۳۸.۳۳%), but BKV was not observed.CONCLUSIONDue to the high prevalence of viral diseases caused by contaminated water and due to the importance of this issue, which will lead to serious risks if health principles are not followed, the presence of BK and JC virus in recreational waters was investigated.

کلیدواژه ها:

Contamination ، recreational waters ، BKV ، JCV