Molecular assessment of recreational water contamination with adenovirus

BACKGROUND AND OBJECTIVESHuman adenoviruses belong to the Adenoviridae family and include more than ۱۰۰ types of adenoviruses. which are divided into seven types based on their biological and biochemical characteristics. They have human diseases, respiratory injuries, gastroenteritis, eye injuries (pictured eye), mango encephalitis and hemorrhagic bladder. A variety of chemical, chemical, and biological contaminants can reduce water quality. Pathogens in water, including viruses, protozoa, pathogens, and a small number of fungi, cause disease. are public While about ۱۵۰ viruses from water are related to human diseases. Human adenoviruses due to their abundant environmental and relative resistance throughout the year, cytopathic development in cultured cells, their appearance similarity to human pathogenic viruses such as rotavirus and norovirus and lower risk for patients. They are considered as a suitable model for the evaluation of water environmental virus.MATERIALS AND METHODSResearch method: samples of recreational waters such as swimming pools, rivers, seas, etc. were collected, and water samples were filtered using ۰.۲۲ and ۰.۴۵ paper filters, and the general adenovirus primer was designed. Viruses were used by polymerase chain reaction (PCR).RESULTS AND DISCUSSION۷ samples out of ۶۰ collected samples were infected with adenovirus.CONCLUSIONThe purpose of this study is to investigate the molecular contamination of recreational waters with adenovirus. Considering the resistance of gastrointestinal viruses such as adenovirus to the purification process, there is a need to pay more attention to the evaluation and control of these viruses

کلیدواژه ها:

Human adenoviruses ، recreational waters ، gastrointestinal ، paper filters ، PCR