Molecular characterization of Trichomonads isolated from human& animals hosts in Alborz Province

BACKGROUND AND OBJECTIVESTrichomonads are amitochondrial anaerobic flagellated protists that are either parasites or commensals, generally living in the digestive or genitourinary tract of humans and animals, which are known to be important agents for gastrointestinal, oral, bronchitis and genital infections in humans and animals. Currently, diagnosis of this organism is made using laboratory methods including direct smear, culture medium and molecular methods. The aim of this study was to determine and identify the types of molecular genotypes of human and domestic trichomoniasis isolates by ITS۱ / ۵.۸S / ITS۲ gene in Alborz province.MATERIALS AND METHODSIn this study, ۸۱ positive samples of trichomonads isolated from vaginal secretions, oral secretions and genital secretions (preputial sheath) were collected from humans and animals in Karaj region. The samples were examined using wet spreading, TYM media and PCR. Out of the isolated positive cases, ۲۰ samples were sent for sequencing. Based on the observed results, the size of the proliferative product of this gene was ۳۷۲ base pairs.RESULTS AND DISCUSSIONThe results of this study were analyzed using phylogenetic tree and neighbor-joining. Furthermore, the genotypes of this family and the possible mutations were investigated in this study.CONCLUSIONThe results of this study showed that the ITS۱/۵.۸S/ITS۲ gene isolated from these samples was more than ۹۹% similar to the sequences in the gene bank. The sequence of the ۵/۸SrRNA gene and its surrounding areas of ITS۱ and ITS۲ showed the low polymorphism of this gene. In birds, two genotypes, A and B, in cattle, ‘bovine’ genotype and in humans, no specific genotype was observed.

کلیدواژه ها:

Trichomonads ، ITS۱/۵.۸S/ITS۲ gene ، Phylogenetic analysis ، Alborz Province