The Effect of Microencapsulated Native Lactobacillus on Gut Gram-negtative Bacteria Flora of Diabetic Male Rats

BACKGROUND AND ABJECTIVEDiabetes is a chronic and complex metabolic disorder that is regarded as one of the leading causes of death in the world. This major problem affects the health of millions of people in all age groups in both genders around the world, the outbreak of which reached ۴۹% in ۲۰۱۰ in the age group of ۲۰ to ۷۹. Consequently, it is expected that it will reach ۷۷% by ۲۰۳۰. According to the World Health Organization, around ۱۷۱ million people worldwide develop it, and it is expected this number will rise by ۳۶۶ million by ۲۰۳۰. Therefore, the search for new therapeutic resources and prevention methods is high on the agenda for the researchers. As the use of medications can have serious side effects, it would be beneficial to use natural compounds in foods for treatment. Probiotics have been selected as live microorganisms, which are potentially useful as food supplements for both humans and animals, and are used in human health improvement and disease prevention.MATERIALS AND METHODSIn this study, samples were taken from the intestine of four groups of diabetic male rats and of the control groups. Then, probiotic bacteria and Escherichia coli bacteria were counted in MRS and EMB agar mediumRESULTS AND DISCUSSIONThe results showed that oral administration of lactobacilli in diabetic group was significantly different from that of the diabetic control group in the number of lactobacillus. The number of Escherichia coli was also significantly lower in the treated diabetic group than that in the diabetic control group

کلیدواژه ها:

Diabetes ، Intestinal microbial flora ، Probiotics ، Lactobacillus ، Escherichia coli