Investigation on the antimicrobial effects of nano-size starch biocomposite assimilated cumin essential oil (Bunium persicum) on Salmonella typhimurium, Shigella flexneri and Klebsiella pneumonia in vitro

BACKGROUND AND ABJECTIVEIn recent years, the use of herbal medicine, as a complementary and effective medicine, is increasing worldwide. Today, with the increasing resistance of bacteria to antibiotics, the use of medicinal plants has been welcomed. The aim of this study was to investigate the properties of antibacterial nano-size starch biocomposite assimilated Cumin essential oil in vitro.MATERIALS AND METHODSIn this experimental study, Cumin essential oil purchased from Zarin Golab Company (Kashan, Iran). The components of the essential oil were identified by gas chromatography and mass spectrometry (GC-MS). After preparing essential oil nanoemulsion, the particle size distribution and morphology of nanoemulsion were examined by Dynamic Light Scattering (DLS) and Transmission Electron Microscope (TEM) respectively, and then, its synthesized nano-capsules. Antimicrobial activity against Salmonella typhimurium, Shigella flexneri and Klebsiella pneumoniae, by disc diffusion, minimum inhibitory concentration (MIC), bactericidal (MBC) by microdilution methods and prevention of biofilm formation.RESULTS AND DISCUSSIONThe result of BPEO analysis by GC/MS apparatus showed that the main of composition essential oil were Cumin aldehyde (۳۰.۴۰%), Phenylglycol (۱۸.۹۹%), Ƴ-Terpinene (۱۵.۵۲%) The difference in chemical volatile compositions essential oil appertain to many factors such as, geographical conditions, soil conditions and else. The TEM and DLS gave parallel resulted. NCBP formulation were demonstrated had best effect than free essential oil and nanoemulsion of counterparts. The microbial cell membrane is damaged when the microorganisms are exposed to antimicrobial agents and the changes in the permeability of cell membrane could lead to the leakage of electrolytes from the interior of the bacterial cellsCONCLUSIONWith attention to the antibacterial properties of Bunium persicum, this compounds can be suggested in the treatment of bacterial infections.

کلیدواژه ها:

Nanoemulsion ، Encapsulation ، Cumin essential oil ، Antibacterial activity ، Gas Chromatography.