Introducing the active substance cinnamaldehyde in Cinnamomum verum plant and investigating its antimicrobial properties against pathogenic bacteria

BACKGROUND AND OBJECTIVESThe spread of antibiotic resistance, especially in gram-negative bacteria, has led to the use of the combined effect of antibiotics and obtaining herbal antibiotic compounds in the treatment of infections caused by these bacteria. The purpose of this study is to investigate the effect of the active substance cinnamaldehyde present in the Cinnamomum verum plant on Pseudomonas aeruginosa under in-vitro conditions, to detect and report the antimicrobial and inhibitory effect on P. aeruginosa bacteria.MATERIALS AND METHODSThe active substance cinnamaldehyde was purchased from Sigma-Aldrich. P. aeruginosa isolates were separated from the clinical samples of patients referred to the laboratory of Razi Hospital in Rasht using different biochemical methods as well as Gram staining method. In this research, ۳۰ clinical strains of P. aeruginosa along with the standard strain ATCC۲۷۸۵۳ were investigated. Antibiogram test (Kirby-Bauer) and quantitative minimum concentration test were used to check the antibacterial effects by successive dilutions of MIC and MBC, and finally, the diameters of the lack of growth around the discs impregnated with ۱ mg/ml of the effective herbal substance cinnamaldehyde were measured and recorded with a ruler.RESULTS AND DISCUSSIONIn this research, the minimum diameter of the non-growth halo of the isolates is reported to be ۱۳ and the maximum diameter is ۲۲ mm (average ۱۶.۸۳ mm) and the average of MIC and MBC cinnamaldehyde on the isolates was ۰.۲۶ and ۰.۵۲ mg/ml, respectively.CONCLUSIONThe results of this study showed that cinnamaldehyde can be used as a suitable candidate with good antimicrobial effects in antibacterial drugs and treatment of infections.

کلیدواژه ها:

P. aeruginosa ، cinnamaldehyde ، Cinnamomum ، Antimicrobial Effects ، Infection.