Characterization of antimicrobial activities of Bifidobacterium lactis BB۱۲ and their inhibitory effect against some foodborne pathogens

BACKGROUND AND OBJECTIVESProbiotics, such as Bifidobacterium animalis BB-۱۲, have gained much interest because of their possible health advantages, including their function in reducing the spread of infections that can be found in food. In this study, we examined the effect of the BB-۱۲ strain and its supernatant on Listeria monocytogenes and Salmonella enterica subsp. enterica serotype Typhimurium growth.MATERIALS AND METHODSAgar well diffusion, disk diffusion, minimum inhibitory concentration (MIC) tests and BCA protein test were used to evaluate the probiotic's antimicrobial activity and supernatants protein concentration.RESULTS AND DISCUSSIONThe results showed that the tested pathogens were significantly inhibited by the probiotic (p<۰.۰۵). Additionally, after ۲۴ and ۴۸ hours of incubation, the BB-۱۲ strain supernatant demonstrated significant antibacterial effect against both pathogens (p<۰.۰۵). A BCA protein test on the cell-free supernatant was carried out to investigate the underlying processes, and the results showed that the ۲۴-hour culture supernatant contained a significant quantity of protein. This implies that the protein components in the supernatant could help explain its increased ability to combat infections.CONCLUSIONThe BB-۱۲ strain and its released proteins as a viable strategy to combat foodborne pathogens, hence enhancing food safety and public health, is highlighted by these findings

کلیدواژه ها:

Foodborne Pathogens ، Probiotics ، Cell-Free Supernatant ، Bifidobacterium Animalis BB-۱۲