The effect of gold nanoparticles on ciprofloxacin-resistant Staphylococcus aureus isolates from skin infections in Qom province

BACKGROUND AND OBJECTIVES In the development of multidrug resistance, efflux pumps effectively pump drug compounds out of cells, which results in reduced membrane permeability to drug compounds. This study evaluated the effect of gold nanoparticles on ciprofloxacin-resistant Staphylococcus aureus isolated from burn patients in Qom province, Iran.MATERIALS AND METHODSTo this end, ۸۸ Staphylococcus aureus strains from skin infection samples in Qom province were identified in genotypic and phenotypic manner using different biochemical methods. The antimicrobial effects of the compounds were measured by disc diffusion and MIC methods according to the CLSI protocol.RESULTS AND DISCUSSION Of ۸۸ S. aureus strains tested, ۵۰ (۵۶.۸۱%) were resistant to ciprofloxacin by disc diffusion method and MIC microdilution was done on them. The highest concentration of inhibitor in ۲۰ strains (۴۰%) was obtained at ۱۲۸ μg / ml during microdilution. Gold nanoparticles alone did not have an effect on Staphylococcus aureus growth inhibition, but in microdilution with ciprofloxacin in the presence of nanoparticles, the minimum growth inhibition concentration of strains was half that of Nano-free MIC.CONCLUSION Gold nanoparticles with ciprofloxacin could be used to prevent the expression of pump genes involved in resistance to fluoroquinolone compounds.

کلیدواژه ها:

Staphylococcus Aureus ، Ciprofloxacin ، Gold Nanoparticles ، Minimum Inhibitory Concentration (MIC) ، Multidrug Resistance.