Assessment of Antibiotic Resistance Genes in Escherichia coli Isolates from Hospital Environments

BACKGROUND AND OBJECTIVESAntibiotic resistance increases the prolonged consequences and mortality caused by E. coli infections. The emergence and expression of resistant genes is a potential factor for the stability of this pathogen against antibiotics. Hence, the present study was designed to investigate the prevalence of antibiotic resistance genes in E. coli isolates from Tabriz, Iran.MATERIALS AND METHODSIn this study, ۱۴۱ females and ۷۸ males aged ۱ to ۸۹ from surgery, internal, intensive care unit, and pediatric wards of Tabriz hospitals were enrolled. E. coli was isolated from ۲۱۹ biological samples (urine, blood, wounds, peritoneum, and respiratory tracts) by culture on sheep blood agar or MacConkey agar and microbial detection standards. The genomic DNA was extracted using cetyltrimethylammonium bromide and resistance genes were identified by polymerase chain reaction method.RESULTS AND DISCUSSIONIn the molecular investigation, at least ۴ high risk genes inducing resistant for E. coli isolates were reported in each antibiotic group. The interpretation of data for each of the antibiotic groups indicates that blaCTXM-۱۵ (۷۰%) among positive beta-lactamases, tetB (۳۱.۶%) in tetracycline, fosC (۴۰%) in fosfomycin, OqxB (۳۴%) in fluoroquinolone, ArmA (۱۲.۹۶%) in aminoglycosides, and Sul۱ (۶۹.۵%) in co-trimoxazole combination are genetic indexes of antibiotic resistance.CONCLUSIONThe genes encoding the destructive factors of antibiotics had a significant prevalence in E. coli isolates. The blaCTXM-۱۵ inducing beta-lactam resistance was the most common gene among our clinical isolates. Due to the possibility of development of resistance genes with chromosomal or plasmid origin, the consumption of antibiotics based on approved global standards has a high priority.

کلیدواژه ها:

E. coli ، antibiotic resistant genes ، PCR ، Azerbaijan ، Iran