Phenotypic survey of cultivable gastric bacteria obtained from patients with gastritis

BACKGROUND AND OBJECTIVESGastritis, characterized by inflammation of the stomach lining, is a common gastric disorder with various causes. Recent studies showed that microbiota of the stomach may be one of the important etiologic factors for development of various gastric diseases. In this study, gastric bacteria of five gastritis patients were isolated and initially assessed by conventional macroscopic and microscopic methods.MATERIALS AND METHODSFive gastric biopsies were obtained from gastritis patients. After homogenization and enrichment, different dilutions of each biopsy were prepared and inoculated on MRS, Brain Heart Infusion blood agar and Mueller Hinton agar under anaerobic (using glove box) and aerobic conditions. Incubation was performed at ۲۸°C and ۳۷°C both aerobically and anaerobically. Cultures were examined after ۴۸-۷۲ hours for colony characteristics and bacterial morphology.RESULTS AND DISCUSSIONAt least one distinct colony was isolated from each patient. Totally, ۱۳ bacterial morphology forms were identified; five G+ bacilli, four G + cocci, two G+ coccobacilli, one G- coccobacilli and one G- bacilli. Based on incubation condition, ۹/۱۳ strains were isolated anaerobically and ۴/۱۳ aerobically. Furthermore, ۹/۱۳ isolates observed at ۳۷°C and ۴/۱۳ at ۲۸°C.CONCLUSIONAnalysis of culturable gastric bacteria obtained from patients with gastritis provided primary valuable insights into their morphological characteristics. Further studies using molecular techniques on these patients as well as other dyspeptic patients could elucidate more details about composition of gastric microbiota and its clinical significance. Furthermore, some isolated bacterial species may provide health benefits and therefore could represent future candidates for microbiota modulating therapies.

کلیدواژه ها:

Gastric microbiota ، Gastritis ، morphological characteristics