Culture–dependent comparison of gastric microbiota in patients with chronic gastritis and gastric atrophy

BACKGROUND AND OBJECTIVESChronic gastritis and gastric atrophy, which is characterized by the loss of normal gastric mucosal cells, are two common stages of gastric diseases. The role of gastric microbiota in the pathogenesis of these diseases is still under investigation. In this primary study, the composition of culturable gastric microbiota was compared in patients with chronic gastritis and atrophy using conventional phenotypic methods.MATERIALS AND METHODSGastric biopsy samples were obtained from ۵ patients classified in gastritis and ۵ patients in atrophy groups by pathological survey. After homogenization and initial enrichment, serial dilutions of each biopsy were inoculated on different selective and non-selective media. Incubation was done in two temperature conditions (۲۸°C and ۳۷°C), both aerobically and anaerobically. After ۲۴-۷۲ hours, the grown colonies were examined by microscopic and macroscopic methods.RESULTS AND DISCUSSIONTotally ۲۸ distict bacterial colonies were identified. Most of the bacterial straines were G+ bacilli which were isolated under anaerobic condition. ۱۳ isolates ( ۱۱ G+ and ۲ G-) were obtained from patients with chronic gastritis and ۱۵ isolates (۱۳ G+ and ۲ G-) from atrophic patients. Out of ۱۳ isolates from patients with chronic gastritis, ۸ strains were bacilli and ۵ strains were cocci form. Out of ۱۵ isolates from atrophic patients, ۹ isolates were bacilli and ۶ isolates were cocci forms.CONCLUSIONRecent studies showed that microbiota of the stomach may also influence the development of gastric disorders. In this pilot study, morphologically-different bacteria were isolated from gastritis and atrophic patients. PCR–based identification of isolated bacteria targeting prokaryotic universal ۱۶S rRNA gene will give more clear insight into gastric bacterial populations in these two patients group and their impact on gastric diseases.

کلیدواژه ها:

Gastric atrophy ، Gastritis ، Microbiota