Investigation of antibacterial activity of copper nanoparticles on Staphylococcus aureus isolated from clinical samples in Kerman hospitals

BACKGROUND AND ABJECTIVEStaphylococcus aureus is one of the most important bacteria causing hospital infections, which has become resistant to various antibiotics over time. Staphylococcus aureus is a gram-positive bacterial that can cause disease in humans. Nanoparticles can penetrate into the cracks of small molecules and cause disintegration in them. Therefore, nanoparticles can be considered a new generation of antibiotic. The aim of this study was to investigate the therapeutic effect of copper nanoparticles on Staphylococcus aureus isolated from clinical samples, considering the growth of antibiotic resistance and exploring new approaches in Nano biotechnology.MATERIALS AND METHODSIn this study, ۳۰ strains of Staphylococcus aureus isolated from clinical samples were examined based on catalase, coagulase, and mannitol fermentation tests for identification and diagnostic purposes. The antibacterial effect of copper nanoparticles synthesized by chemical method was investigated in different concentrations on staphylococcus aureus isolates was investigated by disk method. After incubation at ۳۷ ℃ for ۲۴ hours, the sensitivity of bacteria was determined by measuring the diameter of the growth inhibition zone, and the minimum inhibitory concentration by micro titer plate method was determined.RESULTS AND DISCUSSION۶۶% of Staphylococcus aureus isolates were sensitive to the antibiotic gentamicin, and the average minimum inhibitory concentration was found to be ۱۲۸ μg/ml.All isolates of Staphylococcus aureus showed sensitivity to copper nanoparticles, and the average minimum inhibitory concentration was obtained ۶۴ μg/ml.CONCLUSIONThe sensitivity of Staphylococcus aureus to copper nanoparticles is higher than to antibiotics. The effectiveness of copper nanoparticles against clinical strains of Staphylococcus aureus indicates that this substance can be considered as a suitable antimicrobial agent. it is suggested More research should be done.

کلیدواژه ها:

Staphylococcus aureus ، copper nanoparticles ، antibacterial