Evaluation of antibiotic resistance of uropathogenic Escherichia coli isolates obtained from Afzalipour hospital in Kerman, Iran

BACKGROUND AND OBJECTIVESUropathogenic Escherichia coli (UPEC) is the primary cause of urinary tract infection (UTI) in developed countries such as Iran. Important way to control acute urinary tract infections is the accurate antibiotic therapy. An increasing resistance rate relative to the antibiotics recommended by current guidelines for the treatment of UTIs and an increasing number of multidrug resistant UPEC isolates were observed in recent years. Accordingly, the aim of this study was to evaluate the antibiotic resistance of uropathogenic Escherichia coli (E. coli) isolates.MATERIALS AND METHODSUropathogenic Escherichia coli were isolated from clinical samples obtained from Afzalipour hospital of Kerman city during spring of ۲۰۲۳. Standard biochemical tests were used for identification of UPEC. Twelve common antibiotics from different groups were also selected and used. The antibiotic resistance was determined by Kirby-Bauer disk diffusion susceptibility test. The size of the clear zone around the antibiotic disc is used to classify the antibiotic test results as sensitive, intermediate or resistant. Lack of susceptibility to at least each pathogenic agent in three or more chemical classes of antibiotics was recognized as multidrug-resistant (MDR) E. coli.RESULTS AND DISCUSSIONA total of ۳۰ UPEC strains were isolated from all clinical samples. All identified isolates were fermentative gram-negative motile rods with metallic sheen on EMB, positive for catalase, indole and nitrate reduction but negative for oxidase, citrate, urease and gelatin. resistance of isolates to the common antibiotcs was observed as Ampicillin (۷۶%), Ciprofloxacin (۷۰%), Cefazolin (۷۳%), Trimethoprim/sulfamethoxazol (۷۰%), Imipenem (۴۳%), Cefotaxime (۷۰%), Ceftazidime (۵۳%), Cefepim (۴۶%), Gentamicin (۱۳%), Meropenem (۱۳%), Nitrofurantion (۱۰%), Amikacin (۰%). Multidrug resistance (MDR) was also observed as ۵۶.۷%.CONCLUSIONMDR UPEC has become a complex problem in clinical treatment, and it is essential to monitor E. coli resistance from different sites of infections such as UTI. Updated antibiotic resistance data may enable clinicians to have a better recommendations about antibiotic treatments for UTIs.

کلیدواژه ها:

Escherichia coli ، urinary tract infection ، antibiotic resistance ، Multidrug resistant