Antifungal susceptibility pattern of clinical isolates of Candida parapsilosis

BACKGROUND AND OBJECTIVESCandida species is one of the most common types of fungal infection in the world, which canopportunistically cause disease in people if there is a defect in the immune system or mucosaldefense. Candida parapsilosis is a major human pathogen whose prevalence has grownsignificantly in the last two decades. Considering the increasing resistance of this species toantifungals, this study was conducted with the aim of determining the pattern of drugresistance in order to more effectively treat the infections caused by C. parapsilosis.MATERIALS AND METHODSCandida isolates were all cultured on Sabouraud Dextrose Agar medium for ۴۸ h at ۲۸ °Cand then sub-cultured on a chromogenic medium. Final identification was confirmed by PCRRFLP method. Antifungal susceptibility experiment was assessed using CLSI (Clinical andLaboratory Standard Institute) guidelines for determination of MIC (Minimum inhibitoryconcentration) of three antifungal drugs including fluconazole, voriconazole and itraconazole.RESULTS AND DISCUSSIONAccording to the antifungal susceptibility test results, the MIC ranges for fluconazole,voriconazole and itraconazole were ۰.۲۵-۴, ۰.۰۳۱۳-۰.۲۵ and ۲-۱۶ μg/ml, respectively. Theanalysis of MICs showed that most isolates of C. parapsilosis were sensitive to fluconazoleand itraconazole but most of the isolates were resistant to itraconazole.CONCLUSIONAccording to our results, the best antifungal that can be chosen for C. parapsilosis isfluconazole and voriconazole. Itraconazole may not be suitable for the treatment of C.parapsilosis.

کلیدواژه ها:

Candida parapsilosis ، Clinical isolates ، Drug resistance ، MIC