Antimicrobial and cytotoxicity effects of Lactiplantibacillus pentosus cell extract on standard and clinically resistant Pseudomonas aeruginosa and U۸۷ glioblastoma brain cell line

BACKGROUND AND OBJECTIVESNowadays, the use of lactobacillus cell extracts is one of the new strategies, which its biological properties such as antimicrobial, anti-inflammatory, antiviral, anti-immunomodulatory, and anti-cancer activity have been highly regarded. The aims of this study was to investigate the effect of Lactiplantibacillus pentosus cell extract and its effect on standard and clinical Pseudomonas aeruginosa as well as evaluation its anticancer properties against U۸۷ glioblastoma cell line by MTT assay.MATERIALS AND METHODSIn this study, the cytoplasmic extract of L. pentosus was prepared by sonication method. The concentration and protein content of extract were evaluated by Brad ford method and SDS-PAGE respectively. The antimicrobial property of cytoplasmic extract of L. pentosus was investigated by micro dilution broth and minimum inhibitory concentration (MIC) was determined. The cytotoxicity effect of bacterial extract was evaluated by MTT assay. Finally, ANOVA and Tukey analysis was done in statistical study.RESULTS AND DISCUSSIONThe OD of L. pentosus cytoplasmic extract was assessed as ۰.۱۸۹ at ۶۰۰ nm via ELISA reader, and final concentration by Bradford technique was ۱۲.۳۵۷ μg/μl. The approximated protein antent was ۱۸.۴۸۲%. Based on microdilution test, the MIC of L. pentosus extract on clinical P. aeruginosa was ۱۰۰۰ μg/ml. ANOVA analysis on MTT results with different concentrations of cytoplasmic extract, cleared that the percentage of cell culture survival reduced as the concentration of cell extract increases and a significant relationship between bacterial concentration and reduced in cell culture survival was found (P Value <۰.۰۰۰۱).CONCLUSIONIn the study of Parvin et al ۲۰۲۲, the antimicrobial and anti-biofilm properties of L pentosus MSCIN-۲۴ and MSCIN-۲۵ against food spoilage and local pathogens was evaluated. They showed glycolipid biosurfactants obtained from these strains had broad antimicrobial activityagainst local pathogens and food with MIC in the range of ۵ to ۱۵ mg/ml and caused cell disruption. Also, Abd Ellatif SA et al ۲۰۲۲, showed the probiotic effect and anticancer activity of L. plantarum ESSG۱ (MZ۶۸۳۱۹۴.۱) and L. pentosus ESSG۲ (MZ۶۸۳۱۹۵.۱) isolates from dairy products. They revealed that application of these strains will reduce the use of antibiotics in clinical try. In conclusion, in this study, the antibacterial and cytotoxicity effects of L. plantarum cell extract has been evaluated on clinical P. aeruginosa and U۸۷ cell line is in consistent with a number of recent studies.

کلیدواژه ها:

Lactiplantibacillus pentosus ، Pseudomonas aeruginosa ، U۸۷ Cell line