Synergistic Antimicrobial and Antibiofilm Activities of Methanolic and Ethyl Acetate Extracts from Rosa canina Fruits in Combination with Tetracycline against Pseudomonas aeruginosa and Staphylococcus aureus

BACKGROUND AND OBJECTIVES:The rise of antibiotic resistance necessitates the exploration of alternative approaches to combatting microbial infections. Natural products have shown potential as adjunct therapies to enhance the antimicrobial efficacy of conventional antibiotics. This study aimed to evaluate the antimicrobial (MIC and MBC concentrations) and anti-biofilm activities of methanolic and ethyl acetate extracts derived from Rosa canina fruits against Pseudomonas aeruginosa ATCC۲۷۸۵۳ and Staphylococcus aureus ATCC۱۲۶۰۰, both individually and in combination with tetracycline.MATERIALS AND METHODS:Methanolic and ethyl acetate extracts were obtained from Rosa canina fruits using standard extraction techniques. The antimicrobial activity was assessed using the micro broth dilution method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against P. aeruginosa and S. aureus. Additionally, the anti-biofilm activity was quantified using crystal violet staining method. The extracts were then tested in combination with tetracycline to assess any potential synergistic effects using fractional inhibitory concentration determination (FIC).RESULTS AND DISCUSSIONBoth the methanol and ethyl acetate extracts from Rosa canina fruits exhibited mild antibacterial activity against P. aeruginosa and S. aureus. The MIC values for P. aeruginosa were found to be ۸ and ۴ mg/mL for the methanol and ethyl acetate extract, respectively. For S. aureus, the MIC values was ۱۶ mg/mL for the ethyl acetate extracts. Furthermore, the extracts demonstrated potent anti-biofilm activity, inhibiting biofilm formation and eradicating preformed biofilms. When combined with tetracycline, the extracts exhibited synergistic effects, resulting in enhanced antimicrobial and anti-biofilm activities compared to individual treatments.ConclusionThis study highlights the significant antimicrobial and antibiofilm activities of the methanolic and ethyl acetate extracts obtained from Rosa canina fruits against P. aeruginosa ATCC۲۷۸۵۳ and S. aureus ATCC۱۲۶۰۰.

کلیدواژه ها:

Rosa Canina ، Synergy ، Anti-Biofilm ، Antibacterial