Biosynthesis of carbon nanoparticles from pine fruit soot and investigation of its physical, antibacterial and cytotoxic properties

BACKGROUND AND ABJECTIVEGreen synthesis of nanoparticles is simple and biocompatible and can be produced by a wide range of microorganisms and plants. Since the occurrence of drug resistance and its side effects in the treatment of infections have increased in recent years, the aim of this study was to investigate the antibacterial properties and toxicity of carbon nanoparticles synthesized from pine fruits on human skin fibroblast cell line (CRL-۲۵۲۲).MATERIALS AND METHODSGreen synthesis of carbon nanoparticles from pine fruits was performed by burning and crystallization of soot. The morphology and characteristics of the nanoparticles were investigated using scanning electron microscope (SEM) and energy dispersive X-ray spectroscopy (EDAX), respectively. Multi Drug Resistance (MDR) bacteria isolated from a clinical laboratory in the city of Isfahan were analyzed and classified. MIC determination and agar disc diffusion test was used to investigate the antibacterial activity of the synthesized carbon nanoparticles against five MDR (Multi Drug Resistance) bacterial isolates that cause urinary tract infections. Additionally, colorimetric MTT technique was used to examine the cytotoxic effect of the produced carbon nanoparticles on healthy human skin fibroblast cells (CRL-۲۵۲۲).RESULTS AND DISCUSSIONThe synthesized nanoparticles were spherical and according to the morphological findings, had an average size of less than ۱۰۰ nm. The results of EDAX test revealed that carbon made up ۸۷.۰۸% of the produced nanoparticles. ۶۴ samples of the ۱۰۰ bacterial isolates responsible for urinary tract infections were found to be resistant to more than three popular antibiotics and belonging to five different bacterial genera. The highest inhibitory effect was on Escherichia coli isolate at MIC concentration of ۰.۲۵ mg/ml, and the lowest inhibitory effect was on Klebsiella pneumoniae isolate. The findings of MTT test demonstrated that the synthesized carbon nanoparticles did not exhibit cytotoxicity to the cell line CRL-۲۵۲۲ at concentrations less than ۰.۴ mg/ml for ۲۴ hours.CONCLUSIONThe results of this research showed that carbon nanoparticles synthesized from pine fruit, in addition to antibacterial effect, had no toxicity on human skin fibroblast cells in concentrations less than ۰.۴ mg/mL. These nanoparticles have the potential to be considered in clinical research in the treatment of bacterial infections

کلیدواژه ها:

Green synthesized ، Multi Drug Resistance ، Nanocarbon ، Urinary tract infection