Molecular study of Porphyromonas gingivalis Strains with fim A Genotypes in Periodontitis Patients

Porphyromonas gingivalis belongs to the phylum Bacteroidota and is a nonmotile, Gram-negative, rod-shaped, anaerobic, pathogenic bacterium. It forms black colonies on blood agar.. It is found in the oral cavity, where it is implicated in periodontal disease. This study aimed to isolate and identify pathogenic anaerobic Porphyromonas gingivalis from oral cavities of patients with gingivitis and periodontal disease in Misan governorate center, Iraq. Samples were collected from ۵۰ patients aged ۱۱-۷۰, including ۴۳ females and ۵۷% males. Gram-negative pathogenic bacterial isolates were the most frequent. The study also tested the medical sensitivity of some pathogenic bacterial strains against antibiotics such as Cefoxitin, Doxycycline, Ciprofloxacin, and Nalidixic acid. The strains showed ۱۰۰% resistance to the drugs used in the experiment. Porphyromonas gingivalis has filamentous components on its cell surface called fimbriae (FimA), which are believed to be crucial for the colonization and invasion of periodontal tissues. We have shown before that the nucleotide sequences of the fimA gene allow fimA to be divided into four forms (types I to IV).

کلیدواژه ها:

Fimbriae ، Periodontitis ، Polymerase chain reaction ، Porphyromonas gingivalis ، Bacteria ، gingivitis