Nasal Colonization and Antimicrobial Susceptibility Pattern of Staphylococcus Species among Children in Lahore, Pakistan
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 48
فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JMMI-6-4_003
تاریخ نمایه سازی: 29 بهمن 1402
چکیده مقاله:
Introduction: Staphylococcus is a genus of pathogenic bacteria, which asymptomatically colonizes the upper respiratory tract of the human. The incidence of invasive Staphylococcal infections and the disease burden are high among children in South Asia, including Pakistan. This study aims to determine the nasal colonization and antimicrobial susceptibility pattern of Staphylococcus species isolated from preschool children in Lahore, Pakistan. Methods: A community-based study was conducted in two camps named Shah Di Khui and Jeevan Haana in Lahore city. A total of ۱۰۰ nasal samples, were collected from preschool children from lower-middle-class families during January to March ۲۰۱۸. Species identification was performed using the coagulase test, catalase test, and Gram staining. Also, a ۳۷۰ bp fragment of the tuf gene was targetted using specific primers for the genus Staphylococcus. Antibiotic resistance pattern of the isolates was defined by an antibiotic susceptibility test using a series of antibiotic discs. Results: The results of this study indicated the presence of Staphylococcus species, mainly Staphylococcus aureus in more than ۸۵% of the children. PCR amplification of tuf gene confirmed the identity of the S. aureus isolates from the nasal cultures. Many showed resistance resistant to more than two broad-spectrum antibiotics. Conclusion: The prevalence of nasal colonization of S. aureus was more than ۸۵% among preschool children. Most of the isolates were resistant to β-lactam antibiotics.
کلیدواژه ها:
نویسندگان
Amtul jamil Sami
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Madeeha Khalid
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Shajia jabeen
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Sharqa Khalil
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Ghazala Sajjad
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
Rabia Arooj
Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :