Detection of Mutations of Antimutator Gene pfpI in Pseudomonas aeruginosa Species Isolated from Burn Patients in Tehran, Iran
سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 60
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JMMI-7-4_006
تاریخ نمایه سازی: 29 بهمن 1402
چکیده مقاله:
Introduction: Pseudomonas aeruginosa is an opportunistic pathogen of clinical importance, particularly in immunocompromised and burn patients. This bacterium is becoming resistant to many antibiotics via intrinsic or acquired mechanisms. Mutations in anti-mutator genes, such as pfpI, can be a potential intrinsic mechanism of antibiotic resistance. This study aimed to evaluate the possible effects of mutations of this gene on coding proteins of multi-drug resistant P. aeruginosa isolates. Methods: The antibiotic resistance pattern of ۵۰ P. aeruginosa isolates against ۹ anti-pseudomonas antibiotics was determined by the disk diffusion method. PCR, followed by sequencing, detected the mutations in the pfpI gene. The retrieved sequences were translated to the corresponding amino acid sequences using an online protein database. The amino acid sequences in mutated isolates were compared with the reference sequence using a multiple alignment method. Results: Out of ۵۰ isolates, ۴۳ (۸۶%) were resistant to all antibiotics. Sequencing and multiple alignment analyses showed that amino acids in positions ۲۱, ۲۴, and ۵۷ of pfpI gene were changed in resistant isolates, and all these mutations were observed in each isolate. Homology modeling showed that these amino acids were part of a cleft on the protease. The other point mutations resulted in amino acid changes were in positions ۶۷, ۸۳, and ۱۶۵. Conclusion: The data obtained in this study showed that the pfpI gene of P. aeruginosa might have a significant effect on response to antibiotics. Further epidemiologic and comprehensive studies are required to confirm these findings.
کلیدواژه ها:
نویسندگان
Maryam Khalili-Samani
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Mahmood Barati
Department of pharmaceutical biotechnology, school of pharmacy, Shahid Beheshti University of Medical Sciences
Navid Mirmohammadsadegh
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Mohsen Amin
Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Ali Samadikuchaksaraei
Department of Tissue Engineering & Regenerative Medicine, Iran University of Medical Sciences
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :